Sh-sy5y atcc crl-2266
Webof a neuroblastoma cell line different from SH-SY5Y (see Additional file 1, for full list and exclusion details). Cell source and culture conditions The most-reported source for … WebThe cells can be purchased from ATCC (cat# CRL-2266), ECACC (cat# 94030304) or from other labs, eg. Uppsala University or Stockholm University (Sweden). The native Stockholm University cells display a more developed neuronal phenotype as compared to the cells obtained from ECACC. Equipment Fixed Equipment Balance weighing within the 0.0001-1 …
Sh-sy5y atcc crl-2266
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WebJan 5, 2024 · The HeLa, neuroblastoma (SH-SY5Y ATCC CRL 2266), and embryonic kidney (HEK293 ATCC CRL-1573) cell lines were cultured in a 96-well plate with a cell density of … WebSep 1, 2024 · Probably the most broadly used human cell line in research on neuronal cell biology is the SH-SY5Y cell line (ATCC® CRL-2266™). This neuroblastic cell line is a …
WebSH-SY5Y is a thrice-subcloned cell line derived from the SK-N-SH neuroblastoma cell line. It serves as a model for neurodegenerative disorders since the cells can be converted to … WebCharacteristics: There seem to be differences in the retinoic acid (RA)-induced neuronal phenotype of SH-SY5Y cells from ATCC and ECACC. After 5 days of RA treatment, ECACC …
WebApr 10, 2024 · SH-SY5Y cells (a human neuroblastoma cell line; ATCC #CRL-2266) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Nakalai, Japan) containing Ham’s F12 supplemented with 10% fetal bovine serum ... SH-SY5Y/BoDV cells were treated with RA in the presence of the scrambled siRNA (siCont) ... WebAug 12, 2024 · We tested Nsp2Cas9 editing capacity in additional cell lines, including HeLa, HCT116, A375, SH-SY5Y, and mouse N2a cells, and it could also generate indels in these …
WebNeuroblastoma cell line SH-SY5Y . The SH-SY5Y neuroblastoma cells were obtained from ATCC (#CRL-2266). Cells were cultured in DMEM with 10% FBS and 1X Penicillin-Streptomycin on 0.1% gelatin-coated plates, passaged with 0.05% Trypsin and 0.02% EDTA solution. Cells at passage number 3–10 were used for cytotoxicity assays.
WebSH‐SY5Y Background The PC12 cell line originate from human bone marrowtissue. These are epithelial cells from a neuroblastoma patient (female). These cells are cultured in … early black boxersWebMar 16, 2024 · ATCC-derived human SH-SY5Y (ATCC# CRL-2266) neuroblastoma and murine N2A (ATCC# CCL-131) cells were cultured in DMEM/F12 (Gibco), and DMEM … css vertical align text multiple linesWebCell culture protocol for SH-SY5Y neuroblastoma cells (human, ATCC# CRL-2266) Growth medium: is a 1:1 ratio of ATCC-formulated MEM and F12 • Invitrogen MEM (# 11095-080 with L-Glutamate) Additives:- 1. 1x Non Essential amino acids [(100x stock, 1:100dilution)- Cell Gro#25-025-CI] early black country singersWebATCC’s cationic lipid-based transfection reagents provide high transfection efficiencies for a various cell types, including adherent and suspension cell lines, ... SH-SY5Y CRL-2266 … css vertical and horizontal center divWebSH-SY5Y; Neuroblastoma; Human (Homo sapiens) Product Code: CRL-2266 Supplier Name: ATCC: Size: 1ml Extra Notes: Please allow a minimum of 7-10 days for delivery of most … early black female singersWebAug 26, 2013 · In this context, the popular SH-SY5Y neuroblastoma cell line and its use in in vitro systems is described. Originally derived from a metastatic bone tumor biopsy, SH … css vertically alignWebFeb 18, 2024 · SH-SY5Y cells were obtained from ATCC (CRL-2266, female). SH-SY5Y cells were differentiated as previously described ( Ross et al., 1983 ). In brief, cells were grown in DMEM/F12 (GIBCO) with 10% FBS and 1x GlutaMax (GIBCO). css versiones